Serum proteins analysis by capillary electrophoresis
نویسندگان
چکیده
منابع مشابه
Quantitative analysis of serum proteins separated by capillary electrophoresis.
The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samp...
متن کاملAnalysis of micafungin in serum by capillary zone electrophoresis.
A method is developed for measuring the concentration of micafungin, an echinocandin antifungal agent, in serum, using capillary zone electrophoresis. With a 0.1M borate buffer (pH 10.0) as the run buffer, detection is carried out at 200 nm. Pretreatment is performed by adding acetonitrile to serum (1:2) for deproteinization, allowing the supernatant fluid to be taken for measurement. The detec...
متن کاملSerum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system.
We compared the automated Paragon 2000 clinical capillary zone electrophoresis (CZE) system with two manual methods, agarose electrophoresis (AGE) and cellulose acetate electrophoresis (CAE). Reference intervals in healthy adults were determined for each method. When compared with AGE and CAE, CZE gave substantially higher reference values for the alpha1-globulin fraction. With CZE, within-run ...
متن کاملCapillary Electrophoresis of PEGylated Proteins
Capillary electrophoresis has become a well-established technique for evaluating the composition and concentrations of a wide range of protein samples. In addition, capillary electrophoresis can be used to study a variety of protein properties including the net protein charge, the extent of ion binding, and the presence of specific biomolecular interactions. This wide range of applications is p...
متن کاملEvaluation of Acetonitrile Deproteinisation of the Serum Samples for the Analysis of Drugs in Serum Using Capillary Zone Electrophoresis
Deproteinisation with acetonitrile (along with methanol or other reagents) is a useful and rapid technique in analysis of drugs or their metabolites in human serum. In this paper application of this simple technique in biopharmaceutical analysis using capillary electrophoresis (CE) is evaluated. Some drugs with different ionic and protein binding properties were selected and dissolved in human ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Indian Journal of Clinical Biochemistry
سال: 2001
ISSN: 0970-1915,0974-0422
DOI: 10.1007/bf02864856